Measurement of Ag-Ab interactions

A. Immunogenicity determined by: foreignness, size, complexity, genetic factors, mode of Ag administration

B. Antigens can have multiple determinants (epitopes)

C. Scatchard plot of bound/free vs. bound gives KA (slope) and valency (x-icept)

D. Nonlinearity due to heterogeneous mixture of Ab

E. ELISA, RIA, and competitive bindings measure Ag-Ab binding


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